A combined micro-resonance Raman and absorption set-up enabling in vivo studies under varying physiological conditions: The nerve globin in the nerve cord of Aphrodite aculeata.

نویسندگان

  • K Ramser
  • W Wenseleers
  • S Dewilde
  • S Van Doorslaer
  • L Moens
  • D Hanstorp
چکیده

We hereby report on the design of a set-up combining micro-resonance Raman and absorption spectroscopy with a microfluidic system. The set-up enabled us to study the nerve globin of Aphrodite aculeata in the functional isolated nerve cord under varying physiological conditions for extended periods of time. The oxygenation cycle of the organism was triggered by utilizing the microfluidic system that allowed for a fast switch between aerobic and anaerobic conditions. The nerve globin was found to very easily shift from a penta-coordinated high spin ferrous form to the oxy state upon a change from anaerobic to aerobic conditions. The observed fast reaction to varying O(2) concentrations supports an oxygen-carrying and/or -storing function of the nerve globin. In addition, by combining resonance Raman and absorption spectroscopy, the physiological response could be distinguished from light-induced effects.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Membrane fusion/repair in nerve cells: a biophysical application in spinal cord injuries regeneration

Cell membrane has a critical and vital role in functioning and existence of nerve cells that form central nervous system (CNS) in mammals. Disruption of nerve membrane that normally occurs following an accident injuring spinal cord is known to be the major cause of paralysis. In most occasions, spinal cord injuries are not leading to complete cut in spinal cord fibers but are known to cause cru...

متن کامل

Membrane fusion/repair in nerve cells: a biophysical application in spinal cord injuries regeneration

Cell membrane has a critical and vital role in functioning and existence of nerve cells that form central nervous system (CNS) in mammals. Disruption of nerve membrane that normally occurs following an accident injuring spinal cord is known to be the major cause of paralysis. In most occasions, spinal cord injuries are not leading to complete cut in spinal cord fibers but are known to cause cru...

متن کامل

Nicotinomid Adenin Dinucleotide Phosphate-Diaphorase (NADPH-d) Activity and CB-28 kDa Immunoreactivity in Spinal Neurons of Neonatal Rats after a Peripheral Nerve Lesion

Our previous studies have shown that median and ulnar nerve lesion induced calbindin (CB) immunoreactivity in some injured motoneurons in developing rats. Motoneuron death induced by sciatic nerve transection in neonatal rats has been related to induction of neuronal isoform of nitric oxide synthase (nNOS). The present study investigated whether expression of CB and nicotinomid adenin dinucleot...

متن کامل

Acute Combined Median and Radial Nerve Palsies after Distal Humeral Shaft Fracture

We report a case of a 29-year-old man who presented with a distal humeral shaft fracture sustained by blunt trauma. Physicalexamination and nerve conduction study were consistent with injury to the median and radial nerves proximal to theelbow. The patient underwent open reduction and internal fixation of the humeral shaft fracture with neurolysis of the medianand radial nerves. Repeat electrom...

متن کامل

Effect of Human Umbilical Cord Mesenchymal Stem Cells Transplantation on Nerve Fibers of A Rat Model of Endometriosis

Background Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological disorder associated with pelvic pain and infertility. Some researchers have identified nerve fibers in endometriotic lesions in women with endometriosis. Mesenchymal stem cells (MSCs) have attracted interest for their possible use for both cell and gene therapies because of their capacity for self-renewal ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of biochemical and biophysical methods

دوره 70 4  شماره 

صفحات  -

تاریخ انتشار 2007